Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immuno

N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals.With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine the free morphine in urine of abusers.The detection limit was calculated to be 40 ng/mL.Simulated urine samples were analyzed with good recoveries, which showed the feasibility of its application in specific morphine determination in urine of morphine abusers.

作 者： Jian Qiu MI Xiao Hua QI Xin Xiang ZHANG Wen Bao CHANG   作者單位： The Key Lab of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871  刊 名： 中國化學(xué)快報(英文版)  ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS  年，卷(期)： 2004 15(8)  分類號：   關(guān)鍵詞： Polyclonal antibody   morphine   capillary electrophoresis immunoassay (CEIA)   laser-induced fluorescence (LIF)   specific  

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